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Test Code LAB318 Platelet Function Assay (PFA)

Important Note

Testing must be performed within 4 hours of collection.

Keep at room temperature - do not expose to cold air.

Please tag Blue top with an aliquot label on the cap.

Test Name Alias

Platelet Function Assay | Plt Function Assay | PFA | 4323

Collection Instructions

Specimen Collection: Blood

 

Container(s): 4.5 mL Blue top (3.2% Na. Citrate) and Lavender top (EDTA)

Preferred Volume to Collect: Tubes must be full

Capillary collect ok: No

Microtainer acceptable: No

 

Collection Instructions:

  • Please indicate anticoagulant therapy.

  • Detailed instructions: Blue Citrate Tube

  • Non-tramatic venipuncture should be performed using a 21 guage needle.

  • Avoid leaving the tourniquet on the patient’s arm for an extended period prior to collection.
  • If specimen is collected using a line, then 5.0 mL waste is required, this waste can be given back.
  • Tubes must be full. Do not over fill or under fill; look for the fill indicator lines.
  • Gently invert 8 - 10 times to mix anticoagulant with blood.
  • Testing must be performed within 4 hours of collection.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Whole Blood

Centrifuge/Spin: No

Aliquot: No

Processing Instructions:

  • Testing must be performed within 4 hours of collection
  • Do not refrigerate/freeze or centrifuge specimen
  • Please call for STAT courier pick-up and direct specimen to the Spectrum Health Regional Laboratory (35 Michigan, Grand Rapids)

Transport Temperature:

  • Specimen must be kept at ambient/room temperature.

 

Specimen Stability

Ambient:  4 hours (whole blood state)

Refrigerate: not acceptable

Frozen: not acceptable

Laboratory Retention: 24 hours

Test Frequency

Available 24 hours, usual TAT 4 hours.

Reference Range

PFA Collagen/ADP
60 – 110 second(s)

 

PFA Collagen/Epi
80 – 180 second(s)

 

Closure time above the laboratory reference range could reflect reduced platelet function caused by hematocrit levels <35% or platelet counts <150,000/mL

Clinical Information

Mandatory Reflex Information

  • Platelet Function Assay (PFA 100) with Collagen/Epinephrine results greater than 180 seconds, Collagen/ADP test will be performed.

Performing Department

Coagulation

Performing Department Laboratory Location

Spectrum Health Regional Laboratory (SHRL), Grand Rapids, MI

Methodology

The PFA-100® is an instrument and test cartridge system in which the process of platelet adhesion and aggregation is simulated in vitro mimicking injured endothelium. The time necessary to block a membrane coated with collagen and epinephrine or collagen and ADP is measured and called closure time.

CPT

85576 x2

CDM Code

4098454

Interface Order Alias

10766

Mayo Access Code

SHO4323

LOINC

PFA Collagen/Epi: 24471-5

PFA Collagen/ADP: 24472-3

PFA Hematocrit: 4544-3

PFA Platelet Cnt: 777-3

Reviewed Date

7/13/2016