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Test Code LAB7660 Heparin Dependent Antibody.

Test Name Alias

Heparin Dependent Antibody Level | HIT | Heparin PF4 Antibody | 4279

Collection Instructions

Specimen Collection: Blood

 

Container(s): Red top (no additive)

Preferred Volume to Collect: 6.0 mL

Minimum Volume to Collect: 4.0 mL

Neonate Volume to Collect: 4.0 mL

Capillary collect ok? 

Microtainer acceptable?  

 

Collection Instructions:

  • After collection, gently invert tube 8-10 times.
  • Allow specimen to clot for 30 minutes and centrifuge within 2 hours.
  • Specimen should be processed within 2 hours – See Processing Instructions.

Processing Instructions (Laboratory, Outpatient or Off-site collection)

Processed Specimen: Serum

Centrifuge/Spin: Yes

Aliquot: Yes

Processing Instructions:

  • When tubes are clotted (30 minutes), centrifuge specimen.
  • Pipette serum into 2 screw-capped transport tubes (1 mL each) and freeze

Minimum plasma/serum volume: 2.0 mL serum

Transport Temperature: Frozen

Specimen Stability

Ambient: 24 hours

Frozen: 2 months

Laboratory Retention: 2 months

Test Frequency

Available Monday through Friday. Usual TAT 1-3 days.

Specimens received in the Laboratory by 12:00 (noon) will have results reported the same day.

Reference Range

≤0.7

Clinical Information

Patients should be evaluated using the 4T Score before the Heparin Dependent Antibody test is ordered.  Click on this link to see the 4T Score table.

 

A 4T Score of 3 or less is considered indicative of a low probability of heparin induced thrombocytopenia (HIT). The Heparin Dependent Antibody test should NOT be ordered in such patients.  A 4T Score of 4 or higher should be considered indicative of intermediate or high probability of HIT. The Heparin Dependent Antibody test should be ordered only in patients with a 4T Score at or above this level.

Reflex Information

Positive or borderline results will reflex to Serotonin Release Assay

Performing Department

Coagulation

Performing Department Laboratory Location

Spectrum Health Regional Laboratory (SHRL), Grand Rapids, MI

Methodology

Enzyme-Linked Immunosorbent Assay (ELISA)

CPT

86022

CDM Code

4098606

Interface Order Alias

11136

Mayo Access Code

SHO4279

Reviewed Date

7/13/2016